A research group from the Rovira i Virgili University (URV) in Tarragona has developed a biosensor that can immediately detect very low levels of Salmonella typhi, the bacteria that causes typhoid fever. The technique uses carbon nanotubes and synthetic DNA fragments that activate an electric signal when they link up with the pathogen.
An aptamer attached to an electrode coated with single-walled carbon nanotubes interacts selectively with bacteria.
An aptamer attached to an electrode coated with single-walled carbon nanotubes interacts selectively with bacteria. The resulting electrochemical response is highly accurate and reproducible and starts at ultralow bacteria concentrations, providing a simple, selective method for pathogen detection.
“We have developed a new biosensor that can detect extremely low concentrations of bacteria immediately, easily and reliably”, F. Xavier Rius, lead author of the study and a professor in the Chemometrics, Qualimetrics and Nanosensors research group in the Analytical Chemistry and Organic Chemistry Department of the URV, tells SINC.
Rius’ team, jointly led by Jordi Riu, has come up with a technique that can detect extremely low levels of the bacteria Salmonella typhi, which causes typhoid fever. This new biosensor functions using a method, described this month in the scientific journal Angewandte Chemie International Edition (“Immediate Detection of Living Bacteria at Ultralow Concentrations Using a Carbon Nanotube Based Potentiometric Aptasensor”), which involves carbon nanotubes with inbuilt aptamers providing electrochemical readings.
The aptamers are small fragments of artificial DNA or RNA designed to attach themselves specifically to a particular molecule, cell or micro organism, in this case Salmonella. If the bacteria are not present, the aptamers remain on the walls of the carbon nanotubes. However, if they detect bacteria, they become activated and stick to it, and the carbon nanotubes generate an electric signal that is picked up by a simple potentiometer connected to the biosensor.
“The presence of the bacteria sparks a change in the interaction between the aptamers and the nanotubes, which takes place in a few seconds and creates an increase in the voltage of the electrode”, says Rius.
Traditional methods for identifying and measuring micro organisms require one or two days’ analysis. “This technique means small quantities of micro organisms can be detected simply and practically in real time, just the same as measuring the pH of water”, adds the researcher.
This study is part of the international research being carried out to find the most effective and fast ways of detecting all kinds of pathogens. The new biosensor makes it possible to identify a single cell of Salmonella in a five-millilitre sample and can successfully make quantitative measurements of up to 1,000 bacteria per millilitre.